SUPSATU

Urine Saturation, Kidney Stone

EPIC Test Procedure Code: LAB3583

Synonyms:
Supersaturation
Stone Risk
Performing Lab:
Referral Laboratory
Patient Preparation:

X-ray dyes and contrast media will affect uric acid test results.

-If a kidney X-ray with dye or computerized tomography (CT) scan with contrast has been performed, patient should wait a minimum of 1 day before starting collection.

-If a cholangiography (bile duct X-ray) has performed, patient should wait 7 days before starting collection.

-Urine must be collected before tablets have been taken for gallbladder X-ray, otherwise patient should wait 7 days before starting collection.

Required Patient Info:

If patient is younger than 18 years, patient's height in centimeters and weight in kilograms are required.

Container Type:
Plastic urine container (no preservative) - Keep refrigerated during collection.
Specimen Type:

24 hour urine collection

Preferred Volume:
35 mL
Minimum Volume:
25 mL
Collection Procedure:

24 Hour Urine Collections:—Collect as follows:
1. To begin the collection, empty the bladder and discard this urine. Record this time and date as the start of the collection.
2. 24 Hour urine collections should be started Sunday through Thursday.
3. Collect all urine excreted during the next 24 hours in the urine collection container.
4. Exactly 24 hours after the collection was begun, empty the bladder for the last time. Add this urine to the 24 hour urine container. The collection is finished.
5. The container should be refrigerated during and after the 24 hour collection period until it can be brought to Saint Luke’s Regional Laboratories.

Specimen Processing:

Collect a single 24-hour urine collection without preservative. Record the 24-hour urine total volume in Beaker. 

Store and Transport:
Refrigerated
Stability:

Refrigerated: 14 days

Unacceptable Condition:

Specimens with pH above 8 indicate bacterial contamination, and testing will be canceled. (Do not attempt to adjust pH as it will adversely affect results.)

CPT Codes:

82140 - Supsat Ammonia Urine (EAP 30040544)
82340 - Supsat Calcium Urine (EAP 30040531)
82436 - Supsat Chloride Urine (EAP 30040536)
82507 - Supsat Citrate Urine (EAP 30040537) 
82570 - Supsat Creatinine Urine (EAP 30040550)
84540 - Supsat Urea Nitrogen Urine (EAP 30040551)
83735 - Supsat Magnesium Urine (EAP 30040538)
83935 - Supsat Osmolality Urine (EAP 30040542)
83986 - Supsat pH Urine (EAP 30040543)
84105 - Supsat Phosphorus Urine (EAP 30040533) 
84133 - Supsat Potassium Urine (EAP 30040535)
84300 - Supsat Sodium Urine (EAP 30040532)
84392 - Supsat Sulfate Urine (EAP 30040539)
84560 - Supsat Uric Acid Urine (EAP 30040534)
83945 - Supsat Oxalates Urine (EAP 30040664)

Method:
The major analytes evaluated are potassium, calcium, phosphorus, oxalate, uric acid, citrate, magnesium, sodium, chloride, sulfate, ammonium, urea nitrogen and pH. The protein catabolic rate is calculated from urine urea nitrogen using the formula:

Protein catabolic rate (g/day) =[(UUN+4)* 6.25] g

Given the measured urine concentrations of these analytes and the known affinity constants of the ions for each other at the given pH, a computer program (EQUIL2) calculates a supersaturation for each ion pair of interest (eg, calcium oxalate). Results are expressed as a Delta G (DG) value for each ion pair. DG is the Gibbs free energy of transfer from a supersaturated to a saturated solution.
Clinical Significance:
Diagnosis and management of patients with renal lithiasis:

-Predicting the likely composition of the stone, in patients who have a radiopaque stone, for whom stone analysis is not available. This may help in designing a treatment program.

Aiding in identification of specific risk factors for stones using a 24-hour urine collection

Monitoring the effectiveness of therapy by confirming that the crystallization potential has indeed decreased

Evaluating kidney excretion of acid and urine pH

Estimating a patient's protein intake

Lab Personnel

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