RPD

Rapid Progress Dementia Eval, CSF

EPIC Test Procedure Code: LAB7203

Performing Lab:
Referral Laboratory
Required Patient Info:

Collection kits are provided by the laboratory. Please call your on-site lab to obtain kit prior to collection. (Each lab site will obtain kits by placing an order with SLRL who will get the kits from Reference Lab.)

All specimens must be labeled as "CJD Ordered" and have a biohazard label.

Container Type:
2 Sarstedt CSF False Bottom Tubes (provided in collection kit)
Specimen Type:

CSF

Preferred Volume:
2 Sarstedt CSF False Bottom tubes, each containing 1.5 mL to 2.5 mL
Minimum Volume:
2 Sarstedt CSF False Bottom tubes, each containing 1.25 mL
Collection Procedure:

1. Perform lumbar puncture and discard* the first 1 to 2 mL of cerebrospinal fluid (CSF). (*OR use for other testing.)
2. Collect CSF directly into 2 of the Sarstedt CSF collection tubes until each tube is at least 50% full.**    **The Alzheimer's Association consensus protocol for handling of CSF for clinical measurements of Abeta42 and tau recommends using the drip method for CSF collection and directly collecting into a low-bind polypropylene tube. Although some clinicians prefer the syringe pull method due to speed of collection, the drip method reduces the risk of Abeta42 binding to the plastic of any syringe used.
3. Send CSF specimen in original Sarstedt CSF collection tubes. Do not aliquot. These tubes cannot be shared with other tests.
Note: Polystyrene collection tubes are not acceptable. Exposure of CSF to polystyrene tubes may result in falsely low Abeta42 concentrations.

Store and Transport:
Frozen
Stability:

Only stable in Sarstedt CSF False Bottom tubes provided in collection kit

Ambient: 12 hours
Refrigerated: 14 days
Frozen (preferred): 28 days

Unacceptable Condition:

Gross hemolysis, gross lipemia, gross icterus, discolored CSF, specimen not collected in Sarstedt CSF False Bottom tubes provided in collection kit

CPT Codes:
  • 82234 - BETA-AMYLOID; 1-42 (ABETA 42) CSF RPDE (EAP: 30133256)
  • 84393 - TAU PHOSPHORYLATED EA CSF RPDE (EAP: 31033257)
  • 84394 - TOTAL TAU CSF RPDE (EAP: 31033258)
    84999 - RT QUIC RPDE (EAP: 30133285)

     
Method:
Prion Protein: Second-generation Real-Time Quaking-Induced Conversion (RT-QuIC)
Electrochemiluminescent Immunoassay (ECLIA)
Test Includes:
RT-Quic, Abeta42, T Tau, P Tau P Tau/Abeta42 ratio, t-Tau/p-Tau ratio
Clinical Significance:
Evaluation of individuals presenting with rapidly progressive dementia of uncertain disease etiology and a differential diagnosis of Creutzfeldt-Jakob disease and rapidly progressive Alzheimer disease

Lab Personnel

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