LPMGF
Phytohemagglutinin (PHA) Mitogen
EPIC Test Procedure Code: LAB3673
Performing Lab:
Referral Laboratory
Container Type:
Green top (sodium heparin) tube
Specimen Type:
Whole blood
Preferred Volume:
8 mL
Minimum Volume:
8 mL
Collection Procedure:
Specimens must arrive at the testing laboratory within 24 hours of collection. Collect specimens Monday through Thursday ONLY.
Store and Transport:
Room temperature
Stability:
Room temperature - 24 hours
Unacceptable Condition:
1. Specimen in a lithium heparin tube.
2. Specimen not received at room temperature.
3. Specimen greater than 24 hours old when received at the testing laboratory.
CPT Codes:
86353 - Phytohemagglutinin PHA Mitoge (EAP 30021453)
Method:
Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37 degrees C. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, CD69 and intracellular IFN-gamma), and analyzed by flow cytometry.