LYME AB
Lyme Disease Serology w/Reflex
EPIC Test Procedure Code: LAB6632
Synonyms:
Borrelia burgdorferi
B. burgdorferi
Performing Lab:
Referral Laboratory
Container Type:
Red-top tube or gel-barrier tube,
Specimen Type:
Serum or plasma
Preferred Volume:
2 mL
Alternate Specimens:
Lavender (EDTA) or Lithium Heparin green
Minimum Volume:
0.75 mL (Note: This volume does not allow for repeat testing.)
Specimen Processing:
If tube other than a gel-barrier is used, transfer separated serum or plasma to a labeled plastic transport tube. Do not freeze gel-barrier tube (pour off serum first).
Store and Transport:
Room temperature
Stability:
Frozen - 14 days (Freeze/thaw cycles: Stable x3)
Refrigerated - 14 days
Room Temperature - 14 days
Unacceptable Condition:
Gross hemolysis; gross lipemia; samples containing particulate matter or exhibiting obvious microbial contamination
Limitations:
Screening of the general population should not be performed. The positive predictive value depends on the likelihood of Lyme disease being present. Testing should only be performed on patients with clinical symptoms of Lyme disease or when exposure is suspected.
In general, IgM tests should be disregarded if the patient's symptoms have lasted more than 30 days. If the patient has been sick longer than 30 days, only IgG results should be interpreted.
Potential assay interference due to circulating antibodies in patients with Human Ehrlichiosis (HE) and Tick Borne Relapsing Fever (TBRF), Babesiosis, Parvovirus, and EBV infections has been found. Interpret results from these patients with caution.
In general, IgM tests should be disregarded if the patient's symptoms have lasted more than 30 days. If the patient has been sick longer than 30 days, only IgG results should be interpreted.
Potential assay interference due to circulating antibodies in patients with Human Ehrlichiosis (HE) and Tick Borne Relapsing Fever (TBRF), Babesiosis, Parvovirus, and EBV infections has been found. Interpret results from these patients with caution.
CPT Codes:
86618 - Lyme Disease Serology W/Reflex (EAP 30265381)
Possible Reflex: 86618x2-Reflex Labcorp Lyme IgG/IgM (EAP 30265382)
Method:
Chemiluminescent Immunoassay (CLIA)
Clinical Significance:
Aid in the diagnosis of Lyme disease in individuals with clinical signs and symptoms consistent with Lyme disease. Lyme disease should be considered based on the presence of typical signs and symptoms of infection in patients with a history of possible exposure to infected ticks. This panel utilizes FDA-cleared assays following the modified two-tiered testing (MTTT) algorithm.
Notes:
Specimens are tested using an immunoassay measuring combined IgG and IgM antibodies to specific borrelial proteins; if reactive, the specimens are then tested in separate Borrelia burgdorferi IgG and IgM immunoassays.