HGBEP
Hemoglobin Fractionation Cascade
EPIC Test Procedure Code: LAB3443
Synonyms:
Hemoglobin Electrophoresis
Performing Lab:
Referral Laboratory
Required Patient Info:
The patient's age, ethnic background, MCV, transfusion history, and any known hematological data should be stated on the test request form.
Container Type:
Lavender top (EDTA) tube
Specimen Type:
Whole blood
Preferred Volume:
1.5 mL
Minimum Volume:
0.8 mL
Collection Procedure:
To avoid delays in turn around time, submit a separate EDTA tube for each test requesting an EDTA tube.
Store and Transport:
Refrigerated
Stability:
Frozen - 14 days
Refrigerated - 14 days
Room temperature - 14 days
Unacceptable Condition:
Clotted Sample
CPT Codes:
83020 - Hemoglobin Fractination (EAP 30121220)
Possible Reflex: 83021, 85660
Method:
Capillary electrophoresis; high-pressure liquid chromatography (HPLC); Hgb Solubility: Sodium hydrosulfite reduction
Test Includes:
Capillary electrophoresis (CE) is performed initially. If an abnormal hemoglobin is detected, appropriate testing will be performed at an additional charge. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. These tests may include high-pressure liquid chromatography (HPLC) and/or hemoglobin solubility testing.