PORPHOBIL 24U

Porphobilinogen, Quantitative, 24 Hour Urine

EPIC Test Procedure Code: LAB5178

Synonyms:
Porphobilinogen, QNT, 24 Hr Urine
Performing Lab:
Referral Laboratory
Container Type:
Plastic 24 hour urine container with 30 mL of 30% Glacial Acetic Acid (optional preservative, but required if ordered with Δ-ALA)
Specimen Type:

Urine

Preferred Volume:
3 mL (from a 24 hour collection)
Minimum Volume:
1 mL
Collection Procedure:

24 Hour Urine Collections:

1. To begin the collection, empty the bladder and discard this urine. Record this time and date as the start of the collection.
2. 24 hour urine collections should be started Sunday through Thursday.
3. Collect all urine excreted during the next 24 hours in the urine collection container.
4. Exactly 24 hours after the collection was begun, empty the bladder for the last time. Add this urine to the 24 hour urine container. The collection is finished.
5. Avoid contact with metal during the collection.
6. The container should be refrigerated during and after the 24 hour collection period until it can be brought to Saint Luke’s Regional Laboratories.

Specimen Processing:

1. Measure and record the 24 hour urine total volume on the test request form.
2. Mix well.
3. Transfer the urine into an amber plastic transport tube with amber screw cap. (Protect from light!)
4. Label the container with the patient's name, date, and the time the collection started and finished.
5. Freeze the specimen immediately and maintain frozen until tested.
6. If an amber tube and cap are not available, cover a clear plastic transport tube completely from top to bottom with aluminum foil. Identify the specimen with the patient's name, directly on the transport tube and on the outside of the aluminum foil. Secure with tape.
7. To avoid delays in turn around time when requesting multiple tests on frozen samples, submit separate frozen specimens for each test requested.
8. Freeze the urine aliquot immediately and protect from light.

Store and Transport:
Frozen and protected from light
Stability:

24 hour urine collections preserved with 30% Glacial Acetic Acid:
Frozen - 30 days
Refrigerated - 24 hours

Unacceptable Condition:

Specimen not refrigerated during collection, specimen not protected from light, specimen not a 24 hour collection, or urine aliquot specimen not received frozen at testing laboratory.

CPT Codes:

84110 - Porphobilinogen, Qn, 24-HR Ur (EAP 30120570)
81050 - Urine Volume Measurement (EAP 30033360)

Method:
Ion-exchange chromatography/spectrophotometry

Lab Personnel

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