REF MISC
NT-Pro B-Type Natriuretic Peptide, Serum
Synonyms:
N Terminal proBNP
NT Pro BNP
ProBNP (B-Type Natriuretic Peptide)
Performing Lab:
Referral Laboratory
Patient Preparation:
Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Required Patient Info:
This test should ONLY be ordered on STUDY patients!
See LAB PERSONNEL ONLY section for one other exception.
For all other patients see: Brain Natriuretic Peptide (BNP), Plasma; EPIC Test Procedure Code: LAB4785
Container Type:
Serum gel tube or red-top tube
Specimen Type:
Serum
Preferred Volume:
0.5 mL
Minimum Volume:
0.5 mL
Specimen Processing:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.
Store and Transport:
Frozen
Stability:
Frozen (preferred) - 365 days
Refrigerated - 7 days
Unacceptable Condition:
Gross hemolysis
CPT Codes:
83880
Method:
This is an automated, double-incubation sandwich assay. In the first incubation, antigen from the patient specimen reacts with biotinylated monoclonal sheep N-terminal pro brain natriuretic peptide (NT-proBNP) antibody and monoclonal NT-proBNP antibody labeled with ruthenium complex. During the second incubation, streptavidin-labeled microparticles are added, and the resulting complex is bound to the solid-phase via biotin-streptavidin interaction. The resulting reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of an electrode. Unbound substances are washed away. Voltage is then applied to the electrode, which induces chemiluminescent emission that is measured by a photomultiplier. Results are obtained by comparing this measurement against the calibration curve. The Roche E Modular NT-proBNP assay standardization is traceable to an internal Roche standard. Assigned values for calibrators are traceable to this standardization.(Package insert: ProBNP II. Roche Diagnostics; 08/2018)
Reference Ranges:
Reflex Test :