REF MISC
Enteric Pathogens Culture, Feces
Do not use barium or bismuth before specimen collection
Fecal
Para-Pak C and S
1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.
2. Place feces in preservative within 2 hours of collection.
3. Place vial in a sealed plastic bag.
Ambient - 4 days
Refrigerated - 4 days
Specimen must arrive at Mayo within 96 hours of collection.
Unpreserved stool, ECOFIX preservative, Formalin or PVA fixative
patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.
87045-Enteric Pathogens Culture, Stool-with isolation and preliminary examination
87046 x 3-Stool Culture Aerobic Bacteria, each
Possible Reflex tests:
87077-Ident by MALDI-TOF mass spec (if appropriate)
87077-Bacteria Identification (if appropriate)
87153-Aerobe Ident by Sequencing (if appropriate)
87077-Additional Identification Procedure (if appropriate)
87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)
87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)
The fecal specimen is inoculated onto selective media designed to inhibit growth of normal bowel microbiota while
allowing growth of the enteric pathogens. The following media are used: sheep blood agar, Hektoen enteric (HE) agar,
eosin-methylene blue (EMB) agar, Campylobacter agar, cefsulodin-irgasan-novobiocin (CIN) agar, and the enrichment
broth, selenite F. After incubation, suspect colonies are identified using 1 or a combination of the following techniques:
matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, conventional biochemical
tests, carbon source utilization, serologic methods, or nucleic acid sequencing of the 16S ribosomal RNA gene.
Reportable pathogens include Aeromonas, Campylobacter, Salmonella, Shigella, and Yersinia.
Note: This test does not include culture for Escherichia coli O157:H7. All bacterial isolates worked up, to rule in or out as
a pathogen, will be billed for work performed and resources consumed.